| RPTLC AND DERIVATIVE SPECTROPHOTOMETRY FOR THE ANALYSIS OF SELECTED VITAMINS
I. Baranowska* and A. Kądziolka Department of Analytical and General Chemistry, Silesian Technical University, Gliwice, Poland
SUMMARY
Chromatographic systems have been developed for the reversed phase TLC separation of water- and fat-soluble vitamins on RP-18 as stationary phase. A mixture of five water-soluble vitamins (C, nicotinic acid, nicotinamide, B1 and rutin) was separated using water-methanol (5:4) and water-acetic acid (7:1) as mobile phases. A mixture of fat-soluble vitamins (A-acetate, E, E-acetate and D3) was separated using acetonitrile - benzene - chloroform (10:10:1) as mobile phase. Derivative spectrophotometry was applied to the determination of vitamins B1, B6 and A-acetate in mixtures with other vitamins.
تعداد صفحات کتاب : 12 صفحه
حجم فایل : 1.03 مگا بایت
Password: chemistmag.com
|
